[Biospes] Polyclonal Anti- PCNA Antibody

Catalog: BTL1035

Size: 100μg

Host: Rabbit

Reactivity: Human, mouse, rat, monkey, Chinese hamster, dog

Specificity: Detect the endogenous levels of total PCNA protein.

Immunogen: A synthetic peptide corresponding to internal residues of human PCNA.

Recommend Application:

• Western Blot (1:5000-20000)
• Other applications have not been tested.
• The optimal dilutions should be determined by end user.

Storage Instruction:

• Store at -20°C for 1 year.
• Avoid repeated freeze and thaw cycles.

Background:

Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. It induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. And It has to be loaded onto DNA in order to be able to stimulate APEX2.